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Please use this identifier to cite or link to this item: http://repository.uksw.edu/handle/123456789/4679
Title: Saponin Akasia (Acacia auriculiformis A.cunn) sebagai Pembusa Alami Dan Agensia Antibakteri dalam Sabun Cair
Authors: Ariani, Anidya
Keywords: Acacia auriculiformis;Antibacteria activity;Liquid soap;Saponin;Stability of foam
Issue Date: 2013
Publisher: Program Studi Kimia FSM-UKSW
Abstract: The aims of this study are to isolate saponin from Acacia leaf, to determine the foam stability of saponin liquid soap, to determine the antibacteria activity, of various concentrations of acacia saponin extract, and to make the acacia's saponin soap and to test it according to SNI 06-4085 1996standard. Maseration with ethanol was used as extraction method, then the identification of saponin was carried out by Thin Layer Chromatography and Liberman-Burchard test. The isolation of saponin was perfomed by multiple extraction using hexane and n-butanol as solvent. Antibacteria activity assay was carried out by paper disc method, using Escherichia coliandBacillus subtilisas targeted bacteria. The foam stability assay was perfomed by 1% soap solution. Data were analysed by Randomized Completely Block Design using 7 treatments and 4 replications. To compare the means of the diameter inhibition zones means and the foam stability, the Honestly Significant Differences (HSD) were used at 5% significant level. The results of this study show that the means of isolated acacia's saponin yielded 3,43%. Furthermore,the identification of saponin show that triterpenoid is the main type of saponin. The highest foam stability was 88,92% at 20% saponin liquid soap. Saponin extract has a strong effect to B. subtilisat 5% saponin concentration(Clear inhibition zones 12,22mm), while to E.coli at 20% saponin concentration (Clear inhibition zones 10,57mm). Saponin liquid soap has fulfilled the SNI 06-4085-1996 standard.
URI: http://repository.uksw.edu/handle/123456789/4679
Appears in Collections:T1 - Chemistry

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