Pengaruh Genotipe terhadap Pembentukan Sporofit dari Massa Protalus Pakis Emas (Cibotium barometz (L.) J. Sm.) Secara In Vitro

Abstract

The golden chicken fern, Cibotium barometz, is one of an Indonesian tree fern that has been included in the Appendix II of CITES since 1976. Its rhizomes and hairs are an important export commodity used as medicine, but those parts of the plant still harvested from the wild. In order to conserve the species, Bogor Botanic Gardens has been collect and propagate the plant true in vitro spore culture. The best media for spore germination and development of the gametophytes of C. barometz were ½ MS. However, the growth from gametophyte stage to form sporophyte stage is very late. Previous study indicate genotype is one of the important factor that control the sporophyte formation. The aim of this study is to observe sporophyte development of six genotype of C.barometz in vitro. The spore of six genotype of C. barometz (Cb, Cb1, Cb2, Cb3, Cb4, and Cb5) were used as explant using half strength Murashige and Skoog (1/2 MS) basal media with micro nutrition of MS (full, 1/12, ¼, 1/6 and 1/12). Mass prothallus of the spore culture were subculture in ½ MS basal media with various micro nutrient concentration and incubated in room culture to induce sporophyte. The result shows the highist number of sporophyte were Cb1, Cb4 and Cb5 genotypes.