Please use this identifier to cite or link to this item: https://repository.uksw.edu//handle/123456789/25866
Title: Evaluasi Kestabilan Xanthomonas oryzae phages Hasil Isolasi dari Lahan Sawah Kelurahan Pulutan Kecamatan Sidorejo Kota Salatiga pada Berbagai Kondisi pH
Other Titles: The Stability Evaluation of Xanthomonas oryzae phages Isolated from Paddy Field Pulutan Village Sidorejo Sub-District Salatiga City at Various pH
Authors: Widiyatmoko, Eko Wahyu
Keywords: biokontrol;phages;stabilitas phages;Xanthomonas oryzae;Xanthomonas oryzae phages
Issue Date: 5-Aug-2022
Abstract: Xanthomonas oryzae (Xo) merupakan patogen penyakit hawar daun bakteri (HDB) yang dapat mengurangi produksi padi secara nyata. Petani menanggulangi Xodengan bakterisida sintetik, biokontrol, pestisida nabati dan penanaman padi varietas tahan di lapangan, namun belum sepenuhnya berhasil. Berdasarkan latar belakang tersebut perlu adanya solusi untuk mengendalikan Xo dengan mencari musuh alami yaitu virus yang menginfeksi bakteri. Tujuan penelitian ini adalah mendapatkan partikel Xanthomonas oryzae phages dan menguji stabilitas Xo phages terhadap pH. Metode untuk mendapatkan Xo phages dimulai dengan pengambilan sampel tanah, isolasi, purifikasi, dan propagasi. Pengujian stabilitas pH bertujuan untuk mengetahui seberapa banyak titer Xophages dalam merespon suatu cekaman asam dan basa. Perlakuan pH 3 pada sampel P1, P2, dan P3 ketahanan Xo phages rendah ditunjukkan 8.1013 PFU/ml; 10.1013 PFU/ml; dan 4.1013 PFU/ml. Perlakuan pH 7 sampel P1 dan P2 ketahanan Xo phages stabil ditunjukkan 78.1013 PFU/ml dan 26.1013 PFU/ml,sedangkan P3 Xo phages stabil pada pH 9 ditunjukkan 38.1013 PFU/ml. Perlakuan pH 11 sampel P1, P2, dan P3 turun bertahap ditunjukkan 58.1013 PFU/ml; 12.1013 PFU/ml; dan 36.1013 PFU/ml.Lysate P1 dan lysate P3 cukup tahan terhadap pH basa, dibandingkan dengan sampel lysate P2. Pemberian amelioran dan pupuk yang mengandung unsur magnesium di lahan padi akan bersifat basa, sehingga lysate P1 dan P3 diduga memiliki potensi sebagai biokontrol yang sinergis pada kondisi diatas pH 7.
Xanthomonas oryzae (Xo) is a bacterial leaf blight (BLB) pathogen that an significantly reduce paddy production. Farmers overcome Xo with synthethic bactericides, biocontrol, biopesticide, and planting resistant varieties of paddy in the paddy field, but not completely successful. Based on this problem, it is necessary to find a solution to control Xo by discovering the natural enemies such as a virus that infected bacteria named ‘phage’. This study was to obtain Xanthomonas oryzae phages particle and stability test of Xo phages to pH. The method for obtaining Xo phages begins with soil sampling, isolation, purification, and propagation. The pH stability test aims to determine how much the Xophages titer is responding to an acid and base stress. Treatment of pH 3 on samples P1, P2, and P3 low Xo phages resistance was shown to be 8.1013 PFU/ml; 10.1013 PFU/ml; and 4.1013 PFU/ml. Treatment of pH 7 samples P1 and P2 showed stable Xo phages resistance at 78.1013 PFU/ml and 26.1013 PFU/ml, while P3 Xo phages stable at pH 9 showed 38.1013 PFU/ml. Treatment pH 11 samples P1, P2, and P3 decreased gradually shown 58.1013 PFU/ml; 12.1013 PFU/ml; dan 36.1013 PFU/ml. Lysate P1 and lysate P3 is quite resistent to alkaline pH, compared to P2 lysate samples. The application of ameliorants and fertilizer containing magnesium in paddy fields will be alkaline, so that P1 and P3 lysates are thought to have potential as synergistic biocontrols in conditions above pH 7.
URI: https://repository.uksw.edu/handle/123456789/25866
Appears in Collections:T1 - Agroecotechnology

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