Aktivitas Antioksidan dan Komposisi Kimia Minyak Kulit Batang Pulosari (Alyxia reinwardtii Bl.)

Abstract

Kulit batang Pulosari merupakan salah satu bagian tanaman berkhasiat obat dan memiliki aroma khas, banyak digunakan dalam berbagai resep jamu di Indonesia. Tujuan dari penelitian ini adalah untuk menentukan rendemen, sifat fisiko-kimia dan daya antioksidan dari minyak kulit batang Pulosari. Berdasarkan penelitian yang telah dilakukan, hasil minyak kulit batang Pulosari dengan metode maserasi menggunakan n-heksana menghasilkan purata minyak 2,658±0,098%. Sifat fisiko-kimia minyak meliputi: massa jenis, bilangan asam, bilangan peroksida, dan bilangan penyabunan berturut-turut adalah 0,98±0 g/mL; 92,875±4,954 Mek O2/kg; 11,214±0,776 mg KOH/g; dan 105,678±12,921 mg KOH/g. Komposisi senyawa minyak kulit batang Pulosari paling dominan adalah Cyclopentadecanone, 2-hydroxy- (25,97%); Hexadecanoic acid (14,47%); Ethyl Linoleate (9,70%); 2H-1-Benzopyran-2-one (9,52%); dan 1,2-Benzenedicarboxylic acid, mono(2- ethylhexyl) ester (8,34%). Aktivitas antioksidan minyak diuji dengan DPPH menghasilkan nilai IC50 sebesar 296,700 μg/mL dan dikategorikan sangat lemah.

Pulosari bark is one part of the plant that has medicinal properties and a distinctive aroma, it is widely used in various herbal recipes in Indonesia. The purpose of this study was to determine the yield, Physico-chemical properties, and antioxidant power of Pulosari bark oil. Based on there search that has been done, the yield of Pulosari bark oil by maceration method using n-hexane produces an average oil of 2.658 ± 0.098%. The Physico-chemical properties of the oil include density, acid number, peroxide number, and saponification number espectively are 0.98±0 g/mL; 92.875±4.954 Mek O2/kg; 11.214±0.776 mg KOH/g; and 105.678±12.921 mg KOH/g. The most dominant composition of Pulosari stem bark oil compounds are Cyclopentadecanone, 2-hydroxy- (25.97%); Hexadecanoic acid (14.47%); Ethyl Linoleate (9.70%); 2H-1-Benzopyran-2-one (9.52%); and 1,2-Benzenedicarboxylic acid, mono(2-Ethylhexyl) ester (8.34%). The antioxidant activity of the oil tested with DPPH resulted in an IC50 value of 296,700 g/mL and was categorized as very weak.